In addition, the sequentially tumor-selected antibody and antigen retrieval (STAR) method could identify nanobodies that would bind AML, STAR-isolated nanobodies Nb157 are specifically bound to CD13, which is highly expressed in AML cells, and CD13 CAR-T cells potently eliminated AML in vitro and in vivo. The gene discussed is ANPEP; the disease is acute myeloid leukemia.