Analysis of LGR5 and NANOG expression levels by RT-PCR in these tumors revealed that in mice inoculated with tumor cells expressing PC inhibitors, the level of NANOG was reduced by up to 60% and 40% in Spn4A and α1-PDX-expressing cells injected mice, respectively (Figure 5e,f). Here, NANOG is linked to pachyonychia congenita.