LGR5 and malignant colon neoplasm: Therefore, to evaluate the importance of Furin in the expression of LGR5 and NANOG during tumor progression mediated by colorectal cancer cells harboring these mutations, we used the colon cancer cell lines DLD1 with KRAS mutation, HT29 with BRAF mutation, and HCA7 with WT KRAS and BRAF all with genetically silenced Furin gene (CRISPR/Cas9 approach) [34].