To obtain a melanoma mouse model with selective Loxl3 loss of function, conditional knockout mice for Loxl3 (Loxl3loxP) were first generated as described in the Materials and Methods section and Figure 1A. Loxl3loxP mice were then crossed with Tyr::CreERT2; BrafCA; PtenloxP mice [40], and their offspring were intercrossed until appropriate control (Braf Pten L3wt) and experimental (Braf Pten L3loxP) animals were obtained and confirmed by diagnostic PCR (Figure 1B). Here, PTEN is linked to melanoma.