In three different liver cancer cell lines, HepG2, HuH1, and HuH7, siRNA-mediated STAT3 depletion prolonged STAT1 phosphorylation at Tyr701 upon IL-6 treatment compared to Allstars transfection control (Figure 2 for HuH1 and HuH7 and Supplementary Figure S2A for HepG2). Here, STAT3 is linked to liver cancer.