They used Cas9D10A-based genome editing to introduce the suicide gene HSV1-tk, encoding the prodrug-converting enzyme herpes simplex virus type 1 thymidine kinase into the breakpoints of TMEM135-CCDC67 and MAN2A1-FER fusions in human prostate cancer and hepatocellular carcinoma cells, respectively. This evidence concerns the gene TMEM135 and hepatocellular carcinoma.