Next, we assessed whether microbiota depletion influences the process of NAFLD/NASH and found that the plasma concentration of DX-4000–FITC and serum LPS levels were increased in Il17+/+ mice treated with Abx compared with those in untreated Il17+/+ mice (Fig. 6C); however, the DX-4000–FITC and LPS levels were significantly decreased in Il17−/− mice treated with Abx (Fig. 6C). This evidence concerns the gene IL17A and metabolic dysfunction-associated steatohepatitis.