Through ssGSEA analysis, we obtained a better understanding of GBM with different modification patterns and identified a number of malignant hallmarks of tumors associated with a worse prognosis in the CL2 subgroup, including angiogenesis, DNA repair, coagulation, IL -6/JAK/STAT3 signaling, PI3K/AKT/MTOR signaling, and TGFβ signaling. This evidence concerns the gene TGFB1 and glioblastoma.