Macrophage in this cluster had high expression of type I interferon signaling related genes (IFITM3, IFI27, MX1, IFI6, and ISG15) as well as gene features related to immunosuppressive phenotype (APOE, APOC1, S100A9, and GPNMB) (47, 64, 65), whereby participating in tumor immune regulation (66, 67). This evidence concerns the gene ISG15 and neoplasm.