Cluster M1 was significantly enriched in immune-related pathways such as Th1 and Th2 cell differentiation and antigen processing and presentation (Figure 5D), and high expression antigen presents HLA-related genes and inflammation-related genes (C3, IL2RA, LY86, CX3CR1) (51–53), and macrophages can promote tumor cell growth and metastasis by crosstalk with tumor cells through CX3CR1 (51). Here, C3 is linked to neoplasm.