NCOA4 and bacterial infectious disease: In contrast, western blotting and immunofluorescence imaging showed that ferritin protein expression decreased in the RAW264.7 cells at 3-12 h after bacterial infection, then gradually increased at 24 h (Figure 6G-H, Figure S24), indicating that the ferritin-NCOA-4 axis may contribute to iron accumulation in macrophages during the early stages of bacterial invasion.