Western blot analysis results showed that the expression levels of M2 macrophage markers CD206 and CD163 in these macrophages co-cultured with PTEN deletion, mutation, or silenced cells (U87, U251, and LN229shPTEN) were significantly higher compared with those co-cultured with PTEN wild-type glioma cells (LN229shCONT). This evidence concerns the gene PTEN and glioma.