Notably, we validated the changes of the top altered gene using qPCR and found that Fasn and Scd1 were increased and that Tpm3, Myl2, Myl3, Myh7, Tnnt1, and Tnni1 were reduced in the NAFLD group compared with the controls (Figures 3F, G and Supplementary Table S2). The gene discussed is TNNI1; the disease is metabolic dysfunction-associated steatotic liver disease.