In addition, we investigated the uniquely changed myokines in the NASH group and found that Lep, Ly96, Hp, Adipoq, and Serpina3n were uniquely upregulated, while Chad, Cilp2, Wif1, Fetub, Comp, and Mup14 were uniquely downregulated in the NASH group compared with the CON2 groups (Supplementary Table S3), which were potentially affected by NASH progression. The gene discussed is WIF1; the disease is metabolic dysfunction-associated steatohepatitis.