Besides this, we confirmed the top altered genes by qPCR and found that Fasn, Scd1, Adipoq, Lep, and Srebf1 were largely increased, while Tpm3, Myl2, Myl3, Myh7, Tnnt1, and Tnni1 were sharply reduced in the NASH group in comparison with the control group (Figures 4F, G and Supplementary Table S2). This evidence concerns the gene MYL3 and metabolic dysfunction-associated steatohepatitis.