The results of qPCR showed the mRNA level of WWP1 was downregulated in HuCCT1 cells after MYC silencing while that was upregulated in HCCC-9810 cells overexpressed MYC, which indicated that MYC activated the transcription of WWP1 in ICC cells (Fig. 5D). Here, WWP1 is linked to intrahepatic cholangiocarcinoma.