Interestingly, overexpression of WWP1 again in HuCCT1 cells silenced endogenous WWP1 expression (Fig. 2C, D and Fig. S2A) and knocking down the WWP1 expression again in RBE cells overexpressed WWP1 protein (Fig. 3B, C and Fig. S2B) reversed the ability of proliferation in vitro, these rescue experiments further reinforced the important role of WWP1 on the proliferation of ICC cells. This evidence concerns the gene WWP1 and intrahepatic cholangiocarcinoma.