After isolation from tumor vs spleen, a lower fraction of engineered T cells from the tumor produced the effector cytokines interferon-γ (IFNγ) and tumor necrosis factor-α (TNFα) when stimulated with MSLN406-414 peptide ex vivo (figure 3D), consistent with previously reported declining function in the TME.10 The IFP apparently does not sustain effector function in the TME as no differences were detected between TCR1045 and TCR1045/Fas-4-1BBtm T cells in the fraction of cells producing these effector cytokines (figure 3D) or on a per-cell basis (figure 3E). This evidence concerns the gene FAS and neoplasm.