FAS and neoplasm: After tumor nodules were readily detectable by high resolution ultrasound (>6 weeks after tumor injection), mice were treated with cyclophosphamide ≥6 hours to render the host lymphopenic, and then received 1×107 engineered T cells and a vaccine of 5×107 irradiated splenocytes pulsed with MSLN404-416 peptide to enhance engraftment.10 Prior to transfer,>93% of the transduced T cells expressed TCR1045 or TCR1045/Fas-4-1BBtm, as indicated by staining for the Vβ9 chain of TCR1045 (figure 2B).