CD8A and neoplasm: To determine if FasL expression on ID8VEGF tumor cells also induces CD8 T cell death, we co-cultured wild type ID8VEGF cells with P14 CD8 T cells previously activated in vitro with anti-CD3 and CD28 antibodies (±pretreatment with FasL-blocking antibody), and stained for CC3 in T cells 3 days later.