Next, through hematoxylin and eosin (H&E) tissue staining, we found that compared with the control group, tumor tissue in the CR treatment group showed obvious necrotic cells; the subsequent immunohistochemical analysis showed that the number of Ki-67-positive cells was significantly reduced in the CR treatment group, thus indicating that CR had an antiproliferative effect on colon tumor cells (Fig. 2G). The gene discussed is MKI67; the disease is neoplasm.