To verify the postulation that ATF3/CCL2 axis involves in cerebral ischemia via the TLR4/NF‐κB signaling, the protein expression of ATF3, CCL2, TLR4, and NF‐κB‐p65 in brain tissues of rats with cerebral ischemia was measured. This evidence concerns the gene NFKB1 and brain ischemia.