Finally, the 20 cfDNA-specific variants that were detected in at least one other cfDNA or no other sample included likely HCC driver mutations, including CTNNB1 S33C (P2), NRAS Q61R (P8), and ARID1A R727fs and NF1 E2368fs (both P9). Here, CTNNB1 is linked to hepatocellular carcinoma.