NOTCH2 and neoplasm: Variant detection in tumor DNA and plasma was performed using the genome analysis toolkit v4 Mutect2 caller against germline DNA derived from buffy coat.8 Clonal phylogeny between tumor samples were analyzed in “R”9 with package “CLOE.”10 Serial monitoring of plasma by ARID1A c.4381C>T fs variant and NOTCH2 c.7198C>T fs variant mutant TaqMan assays was performed using the BIORAD Q×200 digital droplet polymerase chain reaction (PCR) system (BIORAD).11