Correspondingly, the phosphorylation of AMPK was significantly lower in Sesn2−/− BDL mice than in Sesn2+/+ BDL mice, and the phosphorylation of p70S6K and S6 were significantly higher in Sesn2−/− BDL livers than in Sesn2+/+ BDL livers (Fig. 5G, H), indicating that bile acid-induced Sestrin2 negatively regulates mTORC1 activity during cholestasis. Here, RPS6 is linked to cholestasis.