CD274 and neoplasm: To determine whether immunological memory is induced by combination therapy with anti-TNFR2 and anti-PD-L1 antibodies, tumor-free mice from the initial subcutaneous model produced by removal of the subcutaneous tumors were challenged by implantation of syngeneic PDAC cells from either the same cell line as the first implantation or a different cell line, using age-matched wild-type mice as controls.