As expected, the downregulation of TNFR2 in pancreatic cancer cells and anti-TNFR2 antibody in vitro made the tumor cells less resistant to lymphocytes by decreasing the numbers of inhibitory of T cells, accompanied by increasing the amount of secreted of IFN-γ and TNF-α in the T cell-mediated tumor cell-killing assay (online supplemental figure 6A-G). This evidence concerns the gene TNF and familial pancreatic carcinoma.