These results showed that miR-3189 dramatically repressed luciferase activity of pmirGLO-GLUT3wt containing the miR-3189 binding site from the wild type GLUT3 3′-UTR; however, luciferase activity upon pmirGLO-GLUT3mt did not repress by miR-3189 in 293 T and GBM cells (Fig. 4E), indicating that miR-3189 can significantly inhibit GLUT3 expression via binding to GLUT3 3′-UTR. This evidence concerns the gene SLC2A3 and glioblastoma.