Next, to determine whether HDAC2 knockdown meaningfully inhibit GBM progression in an in vivo preclinical mouse models as in vitro, we orthotopically xenografted DOX-inducible control and DOX-inducible shHDAC2 U87MG cells into immune-deficient BALB/Cnu/nu mice and administrated the drinking water containing doxycycline after 8 days from the experiment starts (Fig. 2G). The gene discussed is HDAC2; the disease is glioblastoma.