SF3B1 and myelodysplastic syndrome: Since SF3B1 mutations affect the splicing machinery, it could be interesting to calculate for each gene the Shannon entropy of transcript isoforms in order to assess the level of “splicing entropy.” In our work, we have used 10× genomics 3′ short-read sequencing, which does not allow optimal characterization of the alternative transcripts, but this may be possible with a technology that couples single-cell genotyping of SF3B1-mutated MDS with long-read transcriptome sequencing [51].