It was found that si-β-catenin further enhanced the role of Berberine in colitis, further increased cell proliferation ability (Figure 9b, Attached Figure 4b), and apoptosis was further reduced (Figure 9c, Attached Figure 4c), and inflammatory cytokines (IL-12, IL-23 and TNF-α) levels in cell supernatant fluid were further decreased (Figures 9d-f, Attached Figures 4d-f), and TER level was further increased (Figure 9g, attached Figure 4g). This evidence concerns the gene TNF and colitis.