To investigate whether macrophage CD146 is involved in the development of obesity, we first performed flow cytometry to evaluate CD146+ macrophages in visceral adipose tissue (VAT) of C57BL/6 mice fed standard chow (ND) or HFD (60% kcal) for 12 weeks (Figure S1A, Supporting Information). The gene discussed is MCAM; the disease is obesity due to melanocortin 4 receptor deficiency.