MCAM and obesity due to melanocortin 4 receptor deficiency: To further evaluate the obesity‐associated expression of CD146 on macrophages, we measured CD146 expression on mouse bone marrow‐derived macrophages (BMDMs) treated with adipocyte culture medium (ACM), which contains a large amount of lipid and cytokine components, and well mimics the microenvironment of adipose tissue.[23, 24] Compared with BMDMs treated with medium control, ACM‐treated BMDMs showed higher levels of CD11c, CD206, and CD146 by flow cytometry analysis (Figure 1I).