To further evaluate the obesity‐associated expression of CD146 on macrophages, we measured CD146 expression on mouse bone marrow‐derived macrophages (BMDMs) treated with adipocyte culture medium (ACM), which contains a large amount of lipid and cytokine components, and well mimics the microenvironment of adipose tissue.[23, 24] Compared with BMDMs treated with medium control, ACM‐treated BMDMs showed higher levels of CD11c, CD206, and CD146 by flow cytometry analysis (Figure 1I). This evidence concerns the gene MRC1 and obesity due to melanocortin 4 receptor deficiency.