To investigate the effect of Abl expression on HCV life cycle, Huh-7.5, Abl− (KO#1 and KO#2), Abl-WT/Abl− (WT#1 and WT#2), and Abl-KD/Abl− (KD#1 and KD#2) cells were infected with cell culture–adapted HCV (HCVcc) (29) at a multiplicity of infection of 5, and the infected cells were fluorescently stained using anticore monoclonal antibody (mAb) and Hoechst (Fig. 2A). This evidence concerns the gene ABL1 and infection.