While certain proteins show increased expression in both parental cell and EV surfaces, a subset of proteins was found to be either pan-EV markers (ITIH4, MFGE8, TF, DSG1, TSPAN14, AZGP1, and IGSF8) or selectively enriched with Myc overexpression in cancer-derived EVs (ANPEP, SLC38A5, FN1, SFRP1, CDH13, THBS1, and CD44). Here, ANPEP is linked to cancer.