ICOS and neoplasm: A dose-dependent increase in the percentages of ICOS+, CD62L–CD44+ TEM and Ki67+ in CD4+ and CD8+ T cells was observed at 120 h in tumor-draining lymph nodes (TDLNs), while tumor-infiltrated lymphocytes (TILs) showed a dose-dependent increase in the percentage of CD8+Ki67+ T cells, a decrease in the percentage of CD4+CD25+Foxp3+ cells (Tregs) and an apparent increase in granzyme B+ (GZMB) (Fig. 4a,b,e for CT26, Fig. 4c,d,f for EMT6 and Extended Data Fig. 4e for CT26).