To assess the functional significance of SIRT6 in NAFLD, we compared the following hepatocytes: vehicle-treated (control), FFA-treated (FFA), MEG3-transfected and FFA-treated (FFA + MEG3), siSIRT6-transfected and FFA-treated (FFA + siSIRT6), and MEG3- and siSIRT6-co-transfected and FFA-treated (FFA + MEG3 + siSIRT6). Here, MEG3 is linked to metabolic dysfunction-associated steatotic liver disease.