The 8–9-month-old TNF-Tg mice used in this study were confirmed to have severe arthritis in their ankles afferent to the PLVs by μCT with significantly reduced talus bone volumes (WT 1.39 ± 0.07 vs TNF-Tg 0.49 ± 0.12 mm3, p < 0.0001; Supp. The gene discussed is TNF; the disease is arthritic joint disease.