To explore the role of p53 in remodelling the lipid profile of pancreatic cancer, we carried out a global untargeted lipidomic profiling in a cell line (KPshp53) derived from mouse PDAC, with the pancreas-specific expression of KRAS (LSL-KRASG12D) and a doxycycline-regulated short hairpin (sh)RNA targeting wild-type p53 expression. This evidence concerns the gene TP53 and familial pancreatic carcinoma.