KRAS and neoplasm: ddPCR of KRAS variants (covering G12A, G12C, G12D, G12R, G12S, G12V, and G13D) by using the primary ovarian tumor, peritoneal dissemination at the initial recurrence, and appendix obtained from autopsy identified that all the three tumors were positive for the KRAS (G12D) variant with allele frequencies of 10.9–11.0% in the ovarian tumor (presumed tumor content ratio at 30% by hematoxylin and eosin staining), 3.0–3.5% in the peritoneal dissemination (presumed tumor content rate at 10%), and 2.1% in the appendiceal tumor (presumed tumor content rate at 10%), respectively (Fig. 4d).