To understand the role of transmembrane mucin Muc4 in CRC initiation and progression, we generated Apc-/-;Muc4-/-;Cdx2P-creERT2 (referred to AMC) and its contemporary littermate control Apc-/-;Cdx2P-creERT2 (AC) mice (Figure 1C). Here, MUC4 is linked to colorectal carcinoma.