Next, to understand the role of SVA in NLRP3-mediated IL-1β production after SVA infection, we first constructed four types of short-hairpin RNA (shRNA) which target NLRP3, as shown in Fig. 2C, number A and C shRNA effectively interfere with the expression of NLRP3 compared with Mock and NLRP3 shRNA negative control (NC) (lane NC). The gene discussed is NLRP3; the disease is infection.