To assess the effect of GPR56 suppression on leukemic cells, we transduced eight AML cell lines and observed that four of the five lines most sensitive to GPR56 suppression harbored mutations in either NPM1 (OCI‐AML3), DNMT3A (OCI‐AML2, OCI‐AML3), FLT3‐ITD (MV4‐11), or had a MECOM/EVI1 overexpression (HNT34), which represent genetic groups that we had previously connected with high GPR56 expression (Pabst et al, 2016) (Appendix Fig S2B, Dataset EV8). This evidence concerns the gene RUNX2 and acute myeloid leukemia.