To gain more insight into the pathways more active in GPR56high AML, we applied the tool ‘GREAT’ (McLean et al, 2010) to the ATAC‐seq data, which identified enrichment for Rho signaling, a well‐established GPR56 downstream pathway (Iguchi et al, 2008), but also pointed toward the Wnt genes CTNNB1 (β‐catenin) and ITF2/TCF4 (Kolligs et al, 2002) contained in “Coregulation of Androgen receptor activity”, as well as “Hh signaling events mediated by Gli proteins” (Chen et al, 2009; Fig 1G, Dataset EV5). Here, GLI1 is linked to acute myeloid leukemia.