We first dissected in individual functional assays including luciferase reporter assays, IF imaging, and xenotransplantation that GPR56 is functionally important for engraftment of human AML cells in immunocompromised mice and activates not only RhoA signaling, as described before (Piao et al, 2004; Singer et al, 2013; Kishore et al, 2016), but also regulates Wnt and Hh signaling, and EMT‐associated gene expression. This evidence concerns the gene RHOA and acute myeloid leukemia.