The cytokines IL-6 and IL-8 (CXCL-8) are further key elements which are able to enhance 3D growth in PC-3 and have both already shown to induce 3D growth in thyroid tumor cells grown under 1g-conditions using the liquid overlay technique (Svejgaard et al., 2015). The gene discussed is CXCL8; the disease is thyroid tumor.