Moreover, lower expression of miR-26a existed in osteosarcoma CSCs, and lentivirus-mediated upregulation of miR-26a reduced the expression of stem cell biomarkers, including SOX2, CD133, OCT3/4, Nanog, and nucleostemin in osteosarcoma cells (Lu et al., 2017). The gene discussed is POU5F1; the disease is osteosarcoma.