On the other hand, combined knockdown of Wee1 and PKMYT1 causes more HeLa cells to enter mitosis with damaged DNA compared to Wee1 knockdown alone (56), PKMYT1 knockdown enhances AZD1775 induced cell killing in cell lines derived from brain metastases (140), and PKMYT1 is essential for cell survival in a subset of glioblastoma cells that have downregulated Wee1 expression (141). This evidence concerns the gene PKMYT1 and glioblastoma.