No BRCA1 alterations were found in 22% of the tumor samples sequenced with the GeneReader, in 10% with the Ion S5TM and in 4% with the MiSeqTM, whereas no BRCA2 alterations were detected in 2% of the tumor samples sequenced with the GeneReader, but not with the Ion S5TM or the MiSeqTM. The gene discussed is BRCA1; the disease is neoplasm.