To verify the effects of NaIO3 and the role of 5-LOX in RPE in vivo, we used a murine model of NaIO3 (intraperitoneal injection 40 mg/kg body weight)-induced acute retinal degeneration using 6-week-old male wild-type C57BL/6JNarl mice. The gene discussed is ALOX5; the disease is retinal degeneration.