After differential analysis, we found that ENO1 was highly expressed in the malignant samples of bladder cancer, suggesting that ENO1 may be regulated by m6A methylation in bladder cancer and further speculated that the ENO1 gene can induce the activity of its enzyme to affect the glycolysis activity of bladder cancer cells, thereby affecting the progression of the cancer of the bladder. The gene discussed is ENO1; the disease is urinary bladder cancer.