Finally, from the GEO validation set GSE40335, which included eight low-grade and eight high-grade BLCA samples, ENO1 and PGM1 were relatively highly expressed in high-grade BLCA samples, further verifying the significant role of ENO1 and PGM1 in the malignant progression of BLCA. This evidence concerns the gene ENO1 and bladder transitional cell carcinoma.