Nicotine (10 μg/kg/day, gavage) significantly reduced the histologic inflammation in colon tissues by reducing the levels of TNFα, IL-1β, and IL-6 and cell apoptosis and induced autophagy via the AMPK–mTOR–P70S6K pathway in DSS-induced adult male C57BL/6 mice to treat colitis, along with enhanced LC3II/LC3I and beclin-1 and decreased p62 protein level (32). This evidence concerns the gene TNF and colitis.