Our results showed that the expression of lipolysis-related protein CES2 was decreased in WD or WD/CCl4-induced mice, and bicyclol reversed CES2 expression to improve NAFLD, which is consistent with the fact that CES2 is reduced in high fat diet-induced obese mice, diabetic db/db mice, and NASH patients, while the liver disease was alleviated after CES2 was restored (Li et al., 2016; Xu et al., 2021). This evidence concerns the gene CES2 and Wilson disease.