In contrast, the effects and mechanisms of berberine on NAFLD were well documented in various models, which were related to the increase of insulin sensitivity, regulation of the AMPK pathway, improvement of mitochondrial function, alleviation of oxidative stress, stabilization of LDLR mRNA, and regulation of gut microenvironment (Zhu et al., 2016). The gene discussed is LDLR; the disease is metabolic dysfunction-associated steatotic liver disease.