During the de novo lipogenesis, acetyl-CoA is carboxylated to malonyl-CoA by the rate-limiting enzyme acetyl-CoA carboxylase (ACC) and subsequently converted by a multi-step reaction into long-chain fatty acid by fatty acid synthase (FAS) (Mashima et al., 2009; Gathercole et al., 2011), while excessive synthesis of TG is one of the main causative factors for NAFLD (Alves-Bezerra and Cohen, 2017). This evidence concerns the gene FAS and metabolic dysfunction-associated steatotic liver disease.