TLR4 and cystic fibrosis: Therefore, we analyzed the expression levels of these genes together with the two proinflammatory markers Tlr4 and Tff1 and observed upregulation in the mouse model of CF-like lung disease compared to wild-type mice (Fig. 2): SftpA nearly 1.5-fold (p = 0.0475), SftpD 1.7-fold (p = 0.0954), Tff1 approximately 40-fold (p = 0.1547), Tff2 4.5-fold (p = 0.0482), and Tlr4 1.5-fold (p = 0.1507).