Epithelial cellular adhesion molecule (EpCAM) positive and cluster of differentiation (CD)133 negative (EpCAM + CD133 −) cancer stem cells as well as EpCAM negative and CD133 negative (EpCAM − CD133 −) adult cancer cells were derived from two human HCC cell lines (Huh7 and HepG2). The gene discussed is PROM1; the disease is cancer.