While we show that in vitro bradyzoites develop tolerance toward the dual alternative NADH dehydrogenase and DHOD inhibitor HDQ43,68 (Fig. S7D), other mETC inhibitors, such as atovaquone appear to have some effect and lead to a decrease in cyst counts in brains of latently infected mice15–17,69. This evidence concerns the gene ENSG00000251605 and cyst.