Results showed that these candidates were directly involved in several important canonical pathways; Leptin Signaling in Obesity, Estrogen Biosynthesis, Androgen Biosynthesis, Sonic Hedgehog Signaling, PDGF Signaling, Human Embryonic Stem Cell Pluripotency, and the pathway for the regulation of several important cytokines like IL-6, IL-8, IL-3, and IL-15 (Table 2, Sup Table 2, and Sup Figs. 10, 11A). This evidence concerns the gene IL6 and obesity due to melanocortin 4 receptor deficiency.