Spermatogenesis (29 cancers), pancreatic beta cells (20 cancers), oxidative phosphorylation (24 cancers), hedgehog signalling (22 cancers), Wnt-beta catenin signalling (22 cancers), peroxisomes (20 cancers), and the G2/M checkpoint (22 cancers) were enriched in most tumours with low pyroptosis levels, which indicated that pyroptosis was negatively associated with these pathways. The gene discussed is CTNNB1; the disease is neoplasm.