SHH and Miyoshi myopathy: To examine whether or not the HH signaling, which has been reported to be involved in gut mesenchymal SM formation of chicks and mice (Kolterud et al., 2009; Mao et al., 2010; Ramalho-Santos et al., 2000), was involved in MM formation, we assessed the changes in the Sonic hedgehog (SHH) expression during the induction of hGO with MM by immunohistochemistry (IHC) and in situ hybridization (ISH).