Among the recorded clinical information, significant differences were found between the CHC and non-CHC group in age (P = 0.028), tumor size (P = 0.001), hepatitis B virus (HBV) infection status (P = 0.002), cirrhosis status (P = 0.001), serum level of AFP ≥20 ng/ml (P = 0.001), simultaneous elevation of AFP and CA19-9 (P = 0.01), serum level of DCP ≥40 mAU/ml (P = 0.014), serum protein electrophoresis (SPE) Alb and α1 level (P = 0.037 and P > 0.001, respectively). The gene discussed is AFP; the disease is cryohydrocytosis.