To explore the effect of TWIST1 or EP300 on apatinib in GC cells, we transfected shRNAs targeting TWIST1 or EP300 into cells overexpressing COL1A2 and verified the transfection efficiency using RT-qPCR and western blot (all p < 0.01) (Figures 7(a) and 7(b)). This evidence concerns the gene EP300 and gastric cancer.