To further verify the effect of TP5 on colon cancer stem cells, we used flow cytometry to detect the expression of cancer stem cell markers CD24, CD44 and CD133, which are surface markers that can be specifically expressed by colon cancer stem cells (Jaggupilli and Elkord, 2012; Sahlberg et al., 2014). The gene discussed is PROM1; the disease is malignant colon neoplasm.