The percentage of Fluo-4 positive cells decreased from 52.0 ± 2.1% to 47.5 ± 3.0%, 43.5 ± 6.7%, 38.3 ± 2.4% and 32.5 ± 0.6% after 24 h of action of TP5 on HCT116 cells with 100, 200, 400 and 800 μM, respectively (Figures 5A,B), indicating that TP5 can concentration dependently reduce the intracellular Ca2+ concentration of HCT116 cells and thus inhibit the properties of cancer stem cells. This evidence concerns the gene TMPO and cancer.