This study might not directly guide the clinical application of TP5 at this time, but offers the possibility of continuing to modify the TP5 sequence to increase its potency to act on cancer stem cells and protect against immune repair, such as through some unnatural amino acid substitutions, cyclization modifications, etc. Therefore, further research on these scientific questions may lay a solid theoretical foundation for the realization of immunomodulatory peptide TP5/TP5 analogs alone or in combination with chemotherapeutic drugs for the clinical treatment of colon cancer. This evidence concerns the gene TMPO and malignant colon neoplasm.